Regulation of nitrite reductase in the denitrifying bacterium Alcaligenes faecalis S-6.
نویسندگان
چکیده
منابع مشابه
PCR detection of genes encoding nitrite reductase in denitrifying bacteria.
Using consensus regions in gene sequences encoding the two forms of nitrite reductase (Nir), a key enzyme in the denitrification pathway, we designed two sets of PCR primers to amplify cd1- and Cu-nir. The primers were evaluated by screening defined denitrifying strains, denitrifying isolates from wastewater treatment plants, and extracts from activated sludge. Sequence relationships of nir gen...
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Abstract Diversity of the functional genes encoding dissimilatory nitrite reductase was investigated for the first time in denitrifying halobenzoate degrading bacteria and in two 4-chlorobenzoate degrading denitrifying consortia. Nitrite reductase genes were PCR-amplified with degenerate primers (specific to the two different types of respiratory nitrite reductase, nirS and nirK), cloned and se...
متن کاملRefinement of the structure of pseudoazurin from Alcaligenes faecalis S-6 at 1.55 A resolution.
The crystal structure of the redox protein pseudoazurin (123 amino acid residues; molecular weight 13,000 daltons) from Alcaligenes faecalis has been refined by fast Fourier restrained least-squares minimization. Cycles of rebuilding were carried out to escape from local minima. Individual isotropic temperature factor values were refined separately for all atoms. The R factor was reduced from 0...
متن کاملA 2.0-A structure of the blue copper protein (cupredoxin) from Alcaligenes faecalis S-6.
The structure of a blue copper protein, cupredoxin, from the potent denitrifying bacterium Alcaligenes faecalis S-6, has been determined and refined against 2 A x-ray diffraction data. The agreement between observed and calculated structure factors is 0.159, and estimated errors in coordinates are 0.09-0.15 A. The protein folds in a beta sandwich similar to plastocyanin and azurin and includes ...
متن کاملDiversity of nitrite reductase genes (nirS) in the denitrifying water column of the coastal Arabian Sea
Denitrification often occurs in the water column, underlying zones of intense productivity and decomposition in upwelling regions. In the denitrifying zone off the southwest coast of India, high concentrations of nitrite (>15 μM) and nitrous oxide (>500 nM) have been reported near the sediment-water interface (<80 m). We investigated the chemical and molecular indicators of denitrification alon...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1981
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.45.23